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In Vitro Evaluation of the Heparin‐Coated Gyro C1E3 Blood Pump
Author(s) -
Muramatsu Kazuaki,
Masuoka Toshio,
Fujisawa Akira
Publication year - 2001
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1046/j.1525-1594.2001.025007585.x
Subject(s) - heparin , fibrin , chemistry , polycarbonate , biomedical engineering , whole blood , chromatography , materials science , nuclear chemistry , biochemistry , surgery , immunology , organic chemistry , medicine , biology
The blood‐contacting surface, mainly composed of polycarbonate, of the Gyro C1E3 pump was modified using plasma glow discharge to introduce a carboxyl functional group, coated with a base layer of polyethyleneimine as a linker, and coupled with heparin by multi‐ionic binding to enhance blood compatibility. A relative surface content of 3.7% sulfur, which demonstrated heparin immobilization on the polycarbonate substrate, was observed on x‐ray photoelectron spectroscopy, and a initial bioactivity of approximately 88.5 ± 7.3 mIU/cm 2 was obtained by the chromogenic method for antifactor Xa assay. Furthermore, in vitro observation of platelet and fibrin adherence using bovine blood under dynamic flow conditions for 6 h revealed that the multi‐ionically heparinized Gyro C1E3 had significantly stronger antithrombogenecity than the noncoated original type which was evaluated as a good hemocompatible blood pump for clinical use. Not only the Gyro C1E3 but also the ionically heparin‐coated Gyro pump are expected to be thromboresistant in clinical use.