Premium
Oxygen Transfer in a Convection‐Enhanced Hollow Fiber Bioartificial Liver
Author(s) -
Hay P.D.,
Veitch A.R.,
Gaylor J.D.S.
Publication year - 2001
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1046/j.1525-1594.2001.025002119.x
Subject(s) - bioartificial liver device , oxygen , mass transfer , chemistry , michaelis–menten kinetics , volumetric flow rate , oxygen transport , chromatography , biophysics , materials science , hepatocyte , biochemistry , thermodynamics , biology , physics , organic chemistry , enzyme assay , in vitro , enzyme
A mathematical model was developed to predict oxygen transport in a hollow fiber bioartificial liver device. The model parameters were taken from the HepatAssist 2000 device, a plasma perfused hollow fiber cartridge with primary hepatocytes seeded in the extracapillary space. Cellular oxygen uptake was based on Michaelis‐Menten kinetics. Oxygen transport due to the convective flow of plasma into the extracapillary space was considered. The effect of modulating several important parameters was investigated, namely, the Michaelis‐Menten constant V m (the maximum oxygen consumption per unit volume of the cell mass), the oxygen partial pressure, the flow rate of the plasma at device inlet, and the permeability of the cell mass contained in the extracapillary space. A computer implementation of the model was used to assess whether a given number of cells could be maintained within such a device. The results suggest that a substantial proportion of the hepatocytes are exposed to hypoxic conditions under which metabolism may be impaired.