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A Bone Replaceable Artificial Bone Substitute: Cytotoxicity, Cell Adhesion, Proliferation, and Alkaline Phosphatase Activity
Author(s) -
Suh Hwal,
Park JongChul,
Han DongWook,
Lee Dong Hee,
Han Chang Dong
Publication year - 2001
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1046/j.1525-1594.2001.025001014.x
Subject(s) - alkaline phosphatase , osteoblast , adhesion , cytotoxicity , chemistry , cell adhesion , apatite , cell growth , biophysics , biochemistry , in vitro , enzyme , mineralogy , organic chemistry , biology
Cellular toxicity, cell adhesion and proliferation, and alkaline phosphatase (ALP) activity were investigated for an artificial bone substitute composed of heated carbonate apatite (CAp) and Type I atelocollagen (AtCol) extracted from bovine tail skins (88/12 in %wt/wt). To enhance the intramolecular crosslinking between collagen molecules, the CAp‐AtCol substitutes were irradiated by ultraviolet rays (wave length 254 nm) at 4°C for 4 h or vacuum dried at 150°C for 2 h. Cytotoxicity tests by a direct contact method and an extract dilution method revealed that the CAp‐AtCol substitutes were cytocompatible for balb 3T3 fibroblasts. Osteoblast adhesion studies demonstrated that the substitute disks composed of 980°C‐heated CAp and AtCol were significantly more adhesive for osteoblasts than those of 1,200°C‐sintered CAp and AtCol (p < 0.05). Proliferation studies showed that the number of osteoblasts grown in the media containing substitutes of 980°C‐heated CAp and AtCol was statistically higher than grown in those of 1,200°C‐sintered CAp and AtCol after 5 days (p < 0.05). It was found that osteoblasts grown in the substitutes of 980°C‐heated CAp and AtCol only expressed similar ALP activity to the controls. These results suggested that the substitutes consisting of 980°C‐heated CAp and AtCol show more favorable interactions with osteoblasts than those of 1,200°C‐sintered CAp and AtCol.