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The Effect of Biofiltration on Red Blood Cells 2.3‐diphosphoglycerate and pH
Author(s) -
Umimoto Koichi,
Hirai Yasuhiro,
Hayashi Toshio,
Tanaka Hiroshi
Publication year - 2000
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1046/j.1525-1594.2000.06557-2.x
Subject(s) - diphosphoglycerate , hemoglobin , chemistry , bicarbonate , metabolic acidosis , acidosis , medicine , intracellular ph , oxygen , oxygen transport , endocrinology , red blood cell , hemodialysis , biochemistry , intracellular , organic chemistry
To investigate the effect of biofiltration (BF) on the ability of blood to supply oxygen to the peripheral tissues, a 2 week crossover study was conducted with bicarbonate hemodialysis (BcHD) and BF using 5 male patients with diabetic renal failure as subjects. BcHD and BF were performed for 4 h and 3.5 h per session, respectively. Blood gases, the pH of red blood cells (RBC‐pH), and 2.3‐diphosphoglycerate in RBC (RBC‐2.3DPG) were measured during each treatment. After a 2 week BF treatment, the plasma HCO 3 − at the beginning of BF was significantly higher than that of BcHD (p < 0.01), and the blood pH improved with an elevated plasma bicarbonate level (p < 0.05). The RBC‐pH at the beginning of BF was higher than that of BcHD (p < 0.05) although the RBC‐pH at the end of both therapies increased to similar levels. The RBC‐2.3DPG during BcHD remained unchanged, but during BF significantly increased (p < 0.05). Metabolic acidosis was significantly improved by BF with its effect reaching to the RBC intracellular level. The improved metabolic acidosis might occur as a result of the increase in RBC‐2.3DPG during BF. This increase in RBC‐2.3DPG has the effect of reducing the affinity of oxygen for hemoglobin and allows more oxygen to be delivered to the peripheral tissues although the increase in RBC‐pH by dialysis restricts the dissociation of oxygen from hemoglobin.