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Ex Vivo Liver Perfusion with Arterial Blood from A Pig with Ischemic Liver Failure
Author(s) -
Nakamura Noboru,
Kamiyama Yasuo,
Takai Soichiro,
Kamiya Tomoo,
Imamura Atsushi,
Matsui Yoichi,
Wei Tu,
Kaibori Masaki,
Satoi Sohei,
Saitoh Takamichi,
Yoshida Hideyuki,
Yoshida Kazumasa,
Kwon AHon
Publication year - 1999
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1046/j.1525-1594.1999.06131.x
Subject(s) - ex vivo , perfusion , in vivo , arterial blood , liver failure , pig liver , ketone bodies , medicine , biology , metabolism , biochemistry , enzyme , microbiology and biotechnology
To simplify liver support using an ex vivo perfused liver, an isolated pig liver was perfused with arterial blood from the recipient pig while monitoring the metabolic capacity of the ex vivo perfused liver. It was possible to perfuse the isolated liver for more than 24 h using arterial blood from a pig with ischemic liver failure. The viability of the isolated liver during support from the liver failure pig was well maintained as evidenced by the high adenylate energy charge (0.815) and a constant ketone body ratio (KBR) of over 1.0 sampled from the hepatic vein. Oxygen consumption (mean, 29.0 μl/min/g of liver) and bile production (mean, 24.2 μl/h/g of liver) were significantly higher in the isolated liver connected to the liver failure pig than in the organ connected to the pig without liver failure (15.5 μl/min/g and 7.3 μl/h/g, respectively). These findings suggest that this liver support system has sufficient metabolic capacity to support a failed liver. Further studies may provide the experimental basis necessary for the clinical application of this device in treatment of patients with acute liver failure.