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Fibroblast proliferation due to exposure to a platelet concentrate in vitro is pH dependent
Author(s) -
Liu Yawei,
Kalén Anders,
Risto Olof,
Wahlström Ola
Publication year - 2002
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1046/j.1524-475x.2002.10510.x
Subject(s) - fibroblast , platelet , in vitro , incubation , chemistry , wound healing , platelet rich plasma , in vivo , growth factor , blot , microbiology and biotechnology , fibroblast growth factor , platelet derived growth factor , biochemistry , andrology , immunology , biology , platelet derived growth factor receptor , medicine , receptor , gene
The influence of platelet‐rich plasma lysates on fibroblast proliferation was studied in culture. Cells were exposed to platelet lysates that had been preincubated at different pHs (5.0, 7.1, and 7.6). Proliferation was evaluated with the MTT assay and incorporation of [ 3 H]thymidine into macromolecules, while type I collagen production was assayed by Western blotting. Enzyme‐linked immunosorbent assays were used to determine platelet‐derived growth factor and transforming growth factor‐β concentrations. Platelets preincubated in an acidic environment (pH 5.0) induced the highest degree of fibroblast proliferation, and the concentration of platelet‐derived growth factor in the different treated lysates was the highest at that particular pH. The concentration of transforming growth factor‐β, however, was lower after incubation at pH 5.0 than at either pH 7.1 or 7.6. These findings may be relevant to normal wound healing in vivo and useful in the treatment of wounds and delayed healing processes. (WOUND REP REG 2002;10:336–340)