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Expression of preadipocyte factor‐1(Pref‐1), a delta‐like protein, in healing mouse ears
Author(s) -
Samulewicz Stefan Jude,
Seitz Alexander,
Clark Lise,
HeberKatz Ellen
Publication year - 2002
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1046/j.1524-475x.2002.10404.x
Subject(s) - blastema , in situ hybridization , regeneration (biology) , immunohistochemistry , biology , cartilage , wound healing , microbiology and biotechnology , messenger rna , anatomy , gene , immunology , genetics
Preadipocyte factor‐1 (Pref‐1), a delta‐like protein containing epidermal growth factor‐repeats, is expressed in proliferating cells in a variety of tissues and is believed to be involved in maintaining the undifferentiated state of these cells. Using microarray analysis, reverse transcriptase‐polymerase chain reaction, in‐situ hybridization, and immunohistochemistry, we have identified Pref‐1 expression in the healing ears of two strains of mice, MRL and C57BL/6. MRL is unusual in that ear punches completely regenerate the ear tissue along with new cartilage with no scarring. Pref‐1 is more highly expressed in the MRL wounds, is uniquely found in a condensation of cells within the regenerating tissue of the blastema, and may contribute to the regenerative capacity of the MRL ear wound. (WOUND REP REG 2002 ;10:215–221)