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Effects of an aqueous extract from the leaves of Chromolaena odorata (Eupolin) on the proliferation of human keratinocytes and on their migration in an in vitro model of reepithelialization
Author(s) -
Phan Thang T.,
Hughes Margaret A.,
Cherry George W.
Publication year - 2001
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1046/j.1524-475x.2001.00305.x
Subject(s) - keratinocyte , chromolaena odorata , hacat , in vitro , wound healing , cell migration , cell growth , chemistry , cell , pharmacology , microbiology and biotechnology , biology , immunology , botany , biochemistry , weed
Eupolin ointment, prepared from the leaves of Chromolaena odorata , has been shown to promote the healing of soft tissue wounds and burns in Vietnam. However, the mechanism by which this agent affects cells involved in the wound healing process is unknown. Cultured human keratinocytes were used in this study to investigate the effects of the Eupolin extract in vitro on processes involved in wound reepithelialization. Keratinocyte proliferation was monitored by a colorimetric assay and migration by the closure of a denuded area scratched in a confluent monolayer. Human keratinocyte proliferation was stimulated by low concentrations of the extract (from 0.1 to 5 μg/ml), cell differentiation by higher concentrations (50 to 300 μg/ml), and migration by intermediate concentrations (5 to 60 μg/ml). The increased proliferation and migration of human keratinocytes observed in vitro might explain, in part, the beneficial effects that have been observed in the clinic.