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Hyaluronic acid in incision wound fluid: A clinical study with the Cellstick® device in children
Author(s) -
Pajulo Olli T.,
Pulkki Kari J.,
Lertola Kaarlo K.,
Alanen Markku S.,
Reunanen Mikko S.,
Virtanen Riitta V.,
MattilaVuori Armi I.,
Viljanto Jouko A.
Publication year - 2001
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1046/j.1524-475x.2001.00200.x
Subject(s) - hyaluronic acid , wound healing , medicine , analysis of variance , surgical wound , surgery , hyaluronidase , anesthesia , chemistry , anatomy , biochemistry , enzyme
When inserted into a human incision wound, the Cellstick® device harvests inflammatory cells and collects wound fluid, reflecting time‐related changes in cell populations and in wound fluid composition. Hyaluronic acid has been postulated to be an important factor in scar reduction in wound healing and in scarless fetal wound healing. The aim of this work was to determine the concentration and variation of hyaluronic acid and proportions of wound cells in closed surgical wounds in children at two time points. The Cellstick® device was inserted subcutaneously into the wound at the end of an elective inguinal hernia operation on 37 healthy boys, and the devices were removed 3±1 or 24±3 hours after surgery. Haluronic acid concentration was measured from the wound fluid and a differential count of the wound cells was performed. There was a significant decrease in hyaluronic acid concentration from 3±1 to 24±3 hours after surgery (p<0.001, Kruskal‐Wallis anova). The variance of hyaluronic acid concentration in wound fluid differed between the wounds at the two time points (p<0.01, Levene test for homogeneity of variance). A positive correlation between hyaluronic acid concentration and patient age (r=0.91, p<0.05, Spearman) at 3±1 hours post surgery and between HA and wound lymphocytes (r=0.38, p<0.05, Spearman) was also found. We conclude that the hyaluronic acid concentration in wound fluid peaks early in children and decreases significantly by 3 to 24 hours after surgery, and the concentrations in the wound fluid of healthy boys are more variable 3 hours than at 24 hours after surgery.