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Acceleration of full‐thickness wound healing in normal rats by the synthetic thrombin peptide, TP508
Author(s) -
Stiernberg Janet,
Norfleet Andrea M,
Redin William R,
Warner W. Scott,
Fritz Richard R,
Carney Darrell H
Publication year - 2000
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1046/j.1524-475x.2000.00204.x
Subject(s) - thrombin , granulation tissue , wound healing , hemostasis , medicine , saline , inflammation , wound closure , angiogenesis , debridement (dental) , surgery , platelet
Thrombin is an essential factor in hemostasis, inflammation, and tissue repair. The synthetic thrombin peptide, TP508, binds to high‐affinity thrombin receptors and mimics cellular effects of thrombin at sites of tissue injury. Treatment of full‐thickness excisional wounds in normal rats with a single topical application of 0.1 μg TP508 (14 pmol/cm 2 ) reproducibly accelerates wound closure, yielding wounds that on average close 39% more than controls by day 7 ( p < 0.001). Wounds treated with 1.0 μg TP508 are 35% and 43% ( p < 0.001) smaller than controls on day 7 and 10, respectively. The early rate of closure is ~40% greater in TP508‐treated than vehicle‐treated wounds (20 versus 14 mm 2 /day) and remains higher through day 7. Breaking strength after closure is slightly greater (15–23%) in wounds treated with TP508 than with saline alone. Histologic comparisons show that TP508 enhances recruitment of inflammatory cells to the wound site within 24 hours post‐injury. TP508 treatment also augments revascularization of injured tissue, as evidenced at day 7 by the larger size of functional vessels in the granulation tissue and by the directed development of blood vessels to wounds. These studies raise the possibility that TP508 may be clinically useful in management of open wounds.