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Effect of a collagen matrix containing epidermal growth factor on wound contraction
Author(s) -
Inoue Masayuki,
Ono Ichiro,
Tateshita Tohru,
Kuroyanagi Yoshimitsu,
Shioya Nobuyuki
Publication year - 1998
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1046/j.1524-475x.1998.60307.x
Subject(s) - contraction (grammar) , epidermal growth factor , wound healing , chemistry , matrix (chemical analysis) , microbiology and biotechnology , medicine , biology , immunology , biochemistry , receptor , chromatography
Excessive wound contraction is known to lead to pathological wound contracture. Using a rabbit model, we applied a bovine type I collagen matrix sponge as a dermal substitute and human epidermal growth factor to full‐thickness excisional wounds. Wound contraction was assessed 14 and 28 days after wounding. It was found that both collagen matrix and epidermal growth factor significantly inhibited wound contraction ( p < 0.001) in all wounds treated with collagen matrix alone or treated with 0.1 and 1 µg of epidermal growth factor 28 days after wounding. Interestingly, the combination of collagen matrix with epidermal growth factor strongly inhibited wound contraction over matrix alone ( p < 0.01 on day 28). Histological analyses showed a regular horizontal arrangement of collagen fibers in the dermis under wounds treated with these substances but not under untreated wounds. Furthermore, using a fibroblast‐populated collagen gel, the direct inhibitory effect of epidermal growth factor on gel contraction by fibroblasts was also observed. Collagen gels without stimulation contracted to 29.5 ± 0.6% of their original size, as determined 6 days after culturing. At 3 days or more, epidermal growth factor inhibited collagen gel contraction by fibroblasts (after 6 days: 34.2 ± 1.8%, p > 0.05; 36.5 ± 2.8%, p < 0.05; and 39.8 ± 2.1%, p < 0.001 at 1, 10, and 100 ng/ml of epidermal growth factor, respectively). In conclusion, collagen matrix and epidermal growth factor, particularly in combination, may be useful in the prevention of wound contracture.