Premium
Differential expression of platelet‐derived growth factor receptor‐β in an aging model of wound repair
Author(s) -
Brucker Michael J.,
Gruskin Elliot,
Farrell Catherine L.,
Siddiqui Aamir,
Mustoe Thomas A.
Publication year - 1996
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1046/j.1524-475x.1996.40208.x
Subject(s) - wound healing , growth factor , receptor , endocrinology , medicine , downregulation and upregulation , ischemia , growth factor receptor , platelet , biology , immunology , gene , biochemistry
Impaired wound healing as a result of age is a well‐documented phenomenon. However, the overall deficit in healing is substantially increased when the healing wound of an aged animal is ischemic. We hypothesized that both of these deficits are cytokine mediated. We have studied the messenger RNA expression of platelet‐derived growth factor receptor‐β, using the rabbit dermal ulcer model of wound repair, in young (3 to 6 months) and aged (48 months and 60 months) rabbits under normal and ischemic conditions. Platelet‐derived growth factor receptor‐β mRNA expression was measured with the use of quantitative reverse transcriptase‐polymerase chain reaction with incorporation of a synthetic, nonhomologous DNA fragment complementary to platelet‐derived growth factor receptor‐β primers as a competitive internal standard. Results in young rabbits showed a large upregulation of platelet‐derived growth factor receptor‐β mRNA expression after wounding. In both aged animal groups, platelet‐derived growth factor receptor‐β expression was found to be significantly decreased in nonischemic wounds relative to young nonischemic controls. Ischemia was found to have little effect on platelet‐derived growth factor receptor‐β mRNA expression in young animals relative to matched controls. However, ischemia induced a large decrease in the platelet‐derived growth factor receptor‐β mRNA levels of wounds of aged animals relative to paired aged nonischemic wounds. Results suggest an age‐related delay in platelet‐derived growth factor receptor‐β mRNA expression in healing wounds, as well as an age‐related decline in responsiveness to confounding ischemia.