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Interstitial fluids associated with wound repair support proliferation but not differentiation of neonatal rat myoblasts in vitro
Author(s) -
Sicard Raymond E.,
Nguyen Linh M. P.
Publication year - 1994
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1046/j.1524-475x.1994.20412.x
Subject(s) - myocyte , regeneration (biology) , skeletal muscle , creatine kinase , wound healing , cell growth , microbiology and biotechnology , biology , chemistry , andrology , medicine , endocrinology , immunology , biochemistry
The ability of wound fluids to support events required for skeletal muscle regeneration was examined. Wound fluids were obtained from polyvinyl alcohol sponges 1, 3, 5, 10, and 15 days after implantation. Neonatal rat L8 myoblasts were used to test the ability of early wound fluids to promote myoblast proliferation and late wound fluids to promote myoblast differentiation—two characteristics deemed critical for effective skeletal muscle regeneration. Early wound fluids (1‐ and 3‐day) stimulated DNA replication by myoblasts, as judged by tritiated thymidine uptake, up to ninefold ( p < 0.05). Later wound fluids (5‐, 10‐, and 15‐day) displayed decreasing ability to stimulate proliferation, with 15‐day wound fluid failing to significantly stimulate proliferation. In contrast, myoblast differentiation, as judged by myotube fusion and creatine kinase activity, was progressively reduced by wound fluids of increasing age. In fact, late wound fluids (5, 10, and 15 days) reduced myotube fusion by 88% to 100% and depressed creatine kinase activity by 60% to 75% ( p < 0.05). Thus, wound fluids from a repair environment appear to support myoblast proliferation early but suppress myoblast differentiation later. These characteristics suggest that the wound repair environment cannot fully support skeletal muscle regeneration.