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Transforming growth factor‐β enhances connective tissue repair in perforated rat mesentery but not peritoneal macrophage chemotaxis
Author(s) -
Franzén Lennart E.,
Schultz Gregory S.
Publication year - 1993
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1046/j.1524-475x.1993.10304.x
Subject(s) - connective tissue , peritoneal cavity , macrophage , peritoneum , mesentery , laparotomy , medicine , transforming growth factor , intraperitoneal injection , wound healing , transforming growth factor beta , growth factor , pathology , andrology , endocrinology , chemistry , immunology , anatomy , surgery , biochemistry , in vitro , receptor
The perforated rat mesentery model was used to study the effect of transforming growth factor‐β (TGF‐β) on connective tissue repair and influx of macrophages into the peritoneal cavity during such repair. Sprague‐Dawley rats were laparotomized, and mesenteric wounds were made with a scalpel. A daily intraperitoneal injection of 0.5 µg TGF‐β was given for either 2 or 4 days. After 1 to 10 days, the animals received an intravenous injection of tritium‐labeled thymidine before decapitation. Macrophages were collected by peritoneal washing, and the number of closed perforations was counted. Peritoneal cells were quantitated and a labeling index was determined by autoradiography. TGF‐β given for either 2 ( p < 0.001) or 4 ( p < 0.004) days accelerated closure of perforations on days 3 to 7 after injury. Laparotomy as such significantly increased leukocyte influx ( p < 0.004), as well as macrophage‐labeling index ( p < 0.02). However, TGF‐β did not significantly influence either leukocyte influx or macrophage‐labeling index. We concluded that TGF‐β significantly enhances connective tissue repair in this perforated rat mesentery model and that TGF‐β—induced stimulation of repair is not caused by an increased influx of macrophages into the peritoneal cavity.