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Helicobacter ‐induced Gastritis in Mice Not Expressing Metallothionein‐I and II *
Author(s) -
Tran Cuong D.,
Huynh Hien,
Van Den Berg Maartje,
Van Der Pas Mechtelt,
Campbell M. A. Fiona,
Philcox Jeffrey C.,
Coyle Peter,
Rofe Allau M.,
Butler Ross N.
Publication year - 2003
Publication title -
helicobacter
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.206
H-Index - 79
eISSN - 1523-5378
pISSN - 1083-4389
DOI - 10.1046/j.1523-5378.2003.00174.x
Subject(s) - metallothionein , gastritis , helicobacter pylori , gastric mucosa , medicine , biology , helicobacter , gastroenterology , stomach , immunology , pathology , biochemistry , gene
Background. Helicobacter pylori a primary cause of gastritis and peptic ulcer disease, is associated with increased production of reactive oxygen species within the gastric mucosa. Metallothionein (MT), a low‐molecular‐weight, cysteine‐rich, metal‐binding ligand, has been shown to sequester reactive oxygen species and reduce tissue damage. This study investigates the role of MT in H. pylori ‐induced gastritis in mice. Materials and Methods. Control (MT+/+) and MT‐null (MT–/–) mice were inoculated with either 1 × 10 8 H. pylori or H. felis , and were infected for 4, 8 and 16 weeks or 8 weeks, respectively. H. pylori load was determined by culture. Myloperoxidase activity and MT levels were also determined. Results. The stomachs of H. felis ‐infected mice were more severely inflamed than those of H. pylori ‐infected mice. H. felis ‐induced gastritis was more severe ( p = .003) in MT–/– than in MT+/+ mice. MT–/– mice also had higher (60%; p < .05) H. pylori loads than MT+/+ mice 4 weeks after infection but not 8 or 16 weeks after infection. Myloperoxidase activity with H. pylori was similar between MT+/+ and MT–/– mice. Thirty‐three per cent greater ( p < .05) myloperoxidase activity was observed in MT–/– than in MT+/+ mice infected with H. felis . In MT+/+ mice infected with H. pylori , liver MT was increased by 33 and 39% ( p < .05) at 8 and 16 weeks, respectively, whereas gastric MT increased by 46% ( p < .05) at 4 weeks and declined to baseline levels at 8 and 16 weeks. Conclusions. Mice lacking MT are more susceptible to H. pylori colonization and gastric inflammation, indicating that MT may be protective against H. pylori ‐induced gastritis.