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Production of Chemokines and Reactive Oxygen Species by Human Neutrophils Stimulated by Helicobacter pylori
Author(s) -
Shimoyama Tadashi,
Fukuda Shinsaku,
Liu Qiang,
Nakaji Shigeyuki,
Fukuda Yoshihiro,
Sugawara Kazuo
Publication year - 2002
Publication title -
helicobacter
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.206
H-Index - 79
eISSN - 1523-5378
pISSN - 1083-4389
DOI - 10.1046/j.1523-5378.2002.00077.x
Subject(s) - chemokine , helicobacter pylori , reactive oxygen species , interleukin 8 , antibody , chemistry , chemotaxis , stimulation , immunology , inflammation , microbiology and biotechnology , biology , medicine , biochemistry , endocrinology , receptor
Background. Bacteria have different characteristics in stimulation of human neutrophils to produce reactive oxygen species (ROS) and chemokines. This study examined the ability of Helicobacter pylori to induce production of ROS and chemokines by human neutrophils. Methods. H. pylori strains (1.5 × 10 8 CFU/ml) were cocultured with 5 × 10 4 neutrophils isolated from healthy subjects. Samples were incubated with human serum with or without IgG antibodies to H. pylori . ROS production was measured using luminol‐dependent chemiluminescence (LmCL), and the concentrations of chemokines (IL‐8, RANTES, MIP‐1α and MCP‐1) were measured by ELISA. Results. The mean of the highest LmCL (peak height; PH) value stimulated by H. pylori was 3318 in the absence of serum. PH increased to 4687 when incubated with anti‐ H. pylori antibody‐positive sera ( p < .001) but antibody‐negative sera did not affect LmCL response. The mean final concentration of IL‐8 produced in the absence of serum was 142.6 pg/ml. Increased IL‐8 production was seen by addition of antibody positive serum ( p < .01). IL‐8 production was not significantly correlated with production of ROS. On the other hand, H. pylori stimulation did not induce neutrophil production of RANTES, MIP‐1α or MCP‐1. Conclusions. H. pylori was capable of inducing IL‐8 production by human neutrophils, but not C‐C chemokines. Production of C‐X‐C dominant chemokine by neutrophils is consistent with the pathological characteristics of H. pylori ‐induced gastritis, where persistent neutrophil infiltration is present.