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Differences Among Helicobacter pylori Strains Isolated from Three Different Populations and Demonstrated by Restriction Enzyme Analysis of an Internal Fragment of the Conserved Gene hpa A
Author(s) -
Evans Dolores G.,
Queiroz Dulciene M.M.,
Mendes Edilberto N.,
Svennerholm AnnMarie,
Evans, Jr. Doyle J.
Publication year - 1999
Publication title -
helicobacter
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.206
H-Index - 79
eISSN - 1523-5378
pISSN - 1083-4389
DOI - 10.1046/j.1523-5378.1999.98065.x
Subject(s) - restriction fragment length polymorphism , restriction enzyme , biology , genotype , typing , population , genetics , amplicon , helicobacter pylori , polymerase chain reaction , restriction site , restriction fragment , microbiology and biotechnology , gene , demography , sociology
Background. Our goal was to test the idea that Helicobacter pylori genotypes vary from one population to another. Methods. Analysis of Sau 3A and Hin f I restriction fragment–length polymorphism (RFLP) in a 375‐bp polymerase chain reaction amplicon of hpa A was used to compare 31 H. pylori isolates from a relatively small and genetically homogeneous population (Goteborg, Sweden) with those of large, genetically heterogeneous populations located in two different countries (50 isolates from Houston, TX, and 69 isolates from Minas Gerais, a state in the southeastern region of Brazil). Results. Five different Sau 3A and three different Hin f I restriction patterns were found; different combinations of these comprise 10 different RFLP types, I through X. The RFLP types found in the United States and Brazil collections were very similar, except for two Brazil isolates belonging to type VIII and five Brazil isolates belonging to type X, neither type found in the United States. The overall profile of H. pylori isolates from Sweden was remarkably different, with 18 of 31 (58%) having a new Sau 3A restriction pattern, termed gS; 10 of these 18 isolates had Hin f I restriction pattern E (RFLP type VIII), and 8 had Hin f I restriction pattern F (RFLP type IX). No isolates from Sweden belonged to RFLP type III or type X. Conclusions. RFLP typing of a 375‐bp polymerase chain reaction–amplified DNA fragment of H. pylori hpa A revealed that H. pylori genotypes can and do vary from one population to another. We conclude that the unique RFLP profile shown by the group of H. pylori isolates from Goteborg is the result of a cohort effect in this relatively small, stable, genetically homogeneous population. Also, the overall similarity between RFLP profiles of the H. pylori isolates from Texas and Minas Gerais coincides with the fact that although geographically distanced, these populations are similar in being large, dynamic, and genetically heterogeneous.