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Vasopressin‐induced vasoconstriction is dependent on MAPK erk1/2 phosphorylation
Author(s) -
Streefkerk Jörn O.,
Hoogaars Willem M.H.,
Christoffels Vincent M.,
Sand Carsten,
Pfaffendorf Martin,
Peters Stephan L.M.,
Zwieten Pieter A.
Publication year - 2004
Publication title -
fundamental and clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 73
eISSN - 1472-8206
pISSN - 0767-3981
DOI - 10.1046/j.1472-8206.2003.00221.x
Subject(s) - vasopressin , vasoconstriction , mapk/erk pathway , phosphorylation , kinase , western blot , protein kinase a , medicine , endocrinology , extracellular , chemistry , biology , biochemistry , gene
To investigate the involvement of the mitogen‐activated protein kinase (MAPK) family of extracellular signal‐regulated kinase (ERK) 1 and 2 (MAPK erk1/2 ) in the vasopressin‐mediated vasoconstriction in the rat aorta. Vasopressin‐induced vasoconstriction was measured in isolated rat thoracic aortae in the presence or absence of MAPK erk1/2 kinase (MKK mek1/2 ) inhibitors. Thereafter the MAPK erk1/2 phosphorylation in the rat aorta was quantified using Western blot analysis. Vasopressin (1–300 n m ) induced a concentration‐dependent vasoconstriction, which could be inhibited concentration dependently by the selective MKK mek1/2 inhibitors, PD 98059 (10 and 100 μ m ) and U 0126 (10 and 100 μ m ). Western blot analysis revealed a 2.7 ± 0.6‐fold increase in the MAPK erk1/2 phosphorylation induced by vasopressin (300 n m ). This phosphorylation could be dose dependently prevented by both PD 98059 (100 μ m ) and U 0126 (10 and 100 μ m ). These results indicate that vasoconstriction induced by vasopressin is partly regulated by the MAPK erk1/2 pathway.