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Study of the mechanism of Flavobacterium sp. for hydrolyzing organophosphate pesticides
Author(s) -
OrtizHernández M.L.,
QuinteroRamírez R.,
NavaOcampo A.A.,
BelloRamírez A.M.
Publication year - 2003
Publication title -
fundamental and clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 73
eISSN - 1472-8206
pISSN - 0767-3981
DOI - 10.1046/j.1472-8206.2003.00202.x
Subject(s) - phorate , chemistry , flavobacterium , phosphamidon , pesticide , dimethoate , organophosphate , paraoxon , parathion , chromatography , parathion methyl , biochemistry , environmental chemistry , organic chemistry , enzyme , pseudomonas , bacteria , biology , genetics , agronomy , acetylcholinesterase
The biotransformation by Flavobacterium sp. of the following organophosphate pesticides was experimentally and theoretically studied: phorate, tetrachlorvinphos, methyl‐parathion, terbufos, trichloronate, ethoprophos, phosphamidon, fenitrothion, dimethoate and DEF. The Flavobacterium sp. ATCC 27551 strain bearing the organophosphate‐degradation gene was used. Bacteria were incubated in the presence of each pesticide for a duration of 7 days. Parent pesticides were identified and quantified by means of a gas‐chromatography mass spectrum system. Activity was considered as the amount ( μ mol) of each pesticide degraded by Flavobacterium sp. Also, structural parameters obtained by means of the CAChe program package for biomolecules, the reactivity index of phosphorus, of oxygen at the P=O function and of sulfur at the P=S function, and lipophilicity (log P oct ) (ALOGPS v. 2.0) were obtained for each pesticide. Pesticides were hydrolyzed at the bond between phosphorous and the heteroatom, producing phosphoric acid and three metabolites. Enzymatic activity was significantly explained by the following multiple linear relationship:Finally, a mechanism of Flavobacterium sp. to hydrolyze pesticides was proposed.