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LmbJ and LmbIH protein levels correlate with lincomycin production in Streptomyces lincolnensis
Author(s) -
Hola K.,
Janata J.,
Kopecky J.,
Spizek J.
Publication year - 2003
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2003.01432.x
Subject(s) - lincomycin , streptomyces , gene , protein biosynthesis , antibiotics , biology , biosynthesis , methyltransferase , function (biology) , gene expression , streptomycetaceae , overproduction , bacteria , biochemistry , actinomycetales , genetics , methylation
Aims: To demonstrate the expression of two overlapping genes lmbJ and lmbIH in Streptomyces lincolnensis and to document LmbJ and LmbIH protein levels during the lincomycin production phase. To analyse presumable function of the LmbIH protein. Methods and Results: Lincomycin production was monitored by thin‐layer chromatography, proteins LmbJ and LmbIH were assayed in the cell‐free extracts of S. lincolnensis by immunodetection. LmbJ occured at stable level (2–4 mg g −1 of total proteins) for a long time period (36–96 h of cultivation) covering the whole production phase. This fairly corresponds to the catalytic function of the protein in the antibiotic biosynthesis (N‐demethyllincomycin methyltransferase). On the contrary, LmbIH reached the detectable level (0·1 and 0·7 mg g −1 ) just for a short period at 60–72 h. Conclusions: The absence of LmbIH protein at a detectable level during the major part of the antibiotic production phase casts doubt on its possible catalytic function. Rather a different connection with the final biosynthetic steps, e.g. regulatory, can be envisaged. Significance and Impact of the Study: Expression of a newly found putative regulatory gene was demonstrated during production of industrial antibiotic, lincomycin.