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Misidentification of Aeromonas veronii biovar sobria as Vibrio alginolyticus by the Vitek system
Author(s) -
Park T.S.,
Oh S.H.,
Lee E.Y.,
Lee T.K.,
Park K.H.,
Figueras M.J.,
Chang C.L.
Publication year - 2003
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2003.01410.x
Subject(s) - aeromonas , vibrio alginolyticus , biovar , microbiology and biotechnology , aeromonas veronii , biology , vibrionaceae , vibrio , bacteria , genetics
Aims: To find the cause of misidentification of aeromonads when using the Vitek system. Methods and Results: Two Aeromonas veronii biovar sobria isolates were misidentified as Vibrio alginolyticus by the Vitek system. Both strains’ identification was confirmed by biochemical testing, API 20E/20NE kits and/or 16S RFLP analysis. Thirty‐one known Aeromonas species were tested by the Vitek system using 0·45 and 0·85% saline in the suspension medium. It was not clear whether low salinity causes misidentification of Aeromonas species more frequently. Conclusions: The specified reaction time may be inappropriately short for some critical biochemical tests of some strains. An ingenious reading strategy regarding incubation time is necessary to improve identification of Aeromonas species by the Vitek system. Significance and Impact of the Study: To our knowledge, this is the first report of misidentification of A. veronii biovar sobria as V. alginolyticus in the Vitek system.