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Detection of Streptococcus anginosus from saliva by real‐time polymerase chain reaction
Author(s) -
Kumagai K.,
Sugano N.,
Takane M.,
Iwasaki H.,
Tanaka H.,
Yoshinuma N.,
Suzuki K.,
Ito K.
Publication year - 2003
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2003.01405.x
Subject(s) - streptococcus anginosus , saliva , polymerase chain reaction , periodontitis , microbiology and biotechnology , medicine , streptococcaceae , real time polymerase chain reaction , streptococcus , dentistry , biology , bacteria , gene , genetics , antibiotics
Aims: The purpose of the present investigation was to assess the salivary levels of Streptococcus anginosus in periodontitis patients. Methods and Results: The salivary levels of Strep. anginosus were assessed using real‐time polymerase chain reaction (PCR). Streptococcus anginosus was detected in 28 of 37 (75·6%) of periodontitis patients and in three of the 20 (15%) healthy subjects. The mean values for bleeding on probing and probing depth in positive patients were statistically higher than those in negative patients. A significant decrease in Strep. anginosus levels was observed after periodontal treatment. Conclusions: Although the levels of Strep. anginosus are extremely low, they may reflect the status of periodontal health. Significance and Impact of the Study: Real‐time PCR is a useful method for obtaining the relative quantities of Strep. anginosus from saliva samples and for monitoring the effect of therapy.