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The Yersinia HPI is present in Serratia liquefaciens isolated from meat
Author(s) -
Olsson C.,
Olofsson T.,
Ahrné S.,
Molin G.
Publication year - 2003
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2003.01387.x
Subject(s) - yersinia , serratia , enterobacteriaceae , biology , microbiology and biotechnology , yersinia enterocolitica , ribotyping , bacteria , shigella , 16s ribosomal rna , salmonella , polymerase chain reaction , gene , escherichia coli , genetics , pseudomonas
Aims: The aim of the study was to screen the Enterobacteriaceae flora of meat for the presence of bacteria harbouring the Yersinia high‐pathogenicity island (HPI). Methods and Results: Bacteria from 29 meat and 29 liver samples were isolated on violet–red bile glucose agar. A total of 197 isolates were screened for the presence of the irp2 gene, encoded within the HPI, by PCR. One isolate that was positive for irp2 gene was also positive for the fyuA , irp1 , ybtP/ybtQ , ybtX/ybtS and int/asn tRNA genes by PCR. The presence of fyuA , irp1 and irp2 genes was confirmed by Southern hybridization. Conclusions: The isolate was identified as Serratia liquefaciens by sequencing of the 16S rRNA gene and by ribotyping. Significance and Impact of the Study: This is the first report of a Serratia harbouring the Yersinia HPI. Serratia is a frequently occurring Enterobacteriaceae genus in chill‐stored meat.