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Direct PCR detection of Escherichia coli O157:H7
Author(s) -
FodeVaughan K.A.,
Maki J.S.,
Benson J.A.,
Collins M.L.P.
Publication year - 2003
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2003.01386.x
Subject(s) - escherichia coli , enterobacteriaceae , microbiology and biotechnology , biology , bacteria , escherichia coli proteins , polymerase chain reaction , gene , genetics
Aims:This paper reports a simple, rapid approach for the detection of Shiga toxin (Stx)‐producing Escherichia coli (STEC). Methods and Results: Direct PCR (DPCR) obviates the need for the recovery of cells from the sample or DNA extraction prior to PCR. Primers specific for Stx‐encoding genes stx1 and stx2 were used in DPCR for the detection of E. coli O157:H7 added to environmental water samples and milk. Conclusions: PCR reactions containing one cell yielded a DPCR product. Significance and Impact of the Study: This should provide an improved method to assess contamination of environmental and other samples by STEC and other pathogens.