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Automated image analysis to improve bead ingestion toxicity test counts in the protozoan Tetrahymena pyriformis
Author(s) -
Dias N.,
Amaral A.L.,
Ferreira E.C.,
Lima N.
Publication year - 2003
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2003.01382.x
Subject(s) - tetrahymena pyriformis , ingestion , biology , bead , tetrahymena , biological system , biochemistry , materials science , composite material
Aims: To improve bead ingestion counts in Tetrahymena pyriformis by automated image analysis as an alternative to direct‐counts. Methods and Results: Fluorescent latex beads were added to T. pyriformis cultures for ingestion tests. The number of beads ingested by 25 cells was counted directly by epifluorescence microscopy and compared with similar data from image analysis. anova indicated that counts were not significantly different ( P < 0·05). The image analysis particularly provided advantages in terms of speed. Conclusions: The image analysis is superior to direct beads counting in T. pyriformis particularly in terms of speed of analysis. Significance and Impact of the Study: The image analysis method is very rapid and will allow many more toxicological analyses to be undertaken with less operator error.