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Protein synthesis inhibitory activity in culture filtrates from new strains of Streptomyces isolated from Brazilian tropical soils
Author(s) -
Reis S.A.,
Costa L.V.,
Cavalcanti E.D.C.,
GiambiagideMarval M.,
Semêdo L.T.A.S.,
Coelho R.R.R.,
Moussatché N.,
Damaso C.R.A.
Publication year - 2003
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2003.01363.x
Subject(s) - streptomyces , biology , protein biosynthesis , eukaryote , biochemistry , streptomycetaceae , rna , cell culture , polyacrylamide gel electrophoresis , uridine , biosynthesis , microbiology and biotechnology , bacteria , gel electrophoresis , actinomycetales , enzyme , gene , genetics , genome
Aims: To investigate the effect of the culture supernatants from three newly isolated Streptomyces strains, 221, 235 and 606 on eukaryotic cells. Methods and Results: Cell lines were treated with the culture filtrates and assayed for protein synthesis by metabolic labelling, followed by sodium dodecyl sulphate‐polyacrylamide gel electrophoresis analysis. RNA synthesis was investigated by [5‐ 3 H]uridine incorporation. The three culture filtrates presented a strong inhibitory activity, reducing total protein synthesis of different eukaryotic cell lines by more than 85%. No effect on cellular RNA synthesis was detected. The culture filtrates did not affect the growth of the prokaryotic cells tested. Conclusions: These new Streptomyces strains, recently isolated from Brazilian tropical soils, produce molecule(s) with inhibitory activity specific to eukaryote protein synthesis. Significance and Impact of the Study: Streptomyces strains 221, 235 and 606, probably representing new species, might produce new bioactive compound(s), and can be used as valuable tools to study the protein synthesis pathway in eukaryotes.