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Two‐dimensional environmental profiles of growth, deoxynivalenol and nivalenol production by Fusarium culmorum on a wheat‐based substrate
Author(s) -
Hope R.,
Magan N.
Publication year - 2003
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2003.01358.x
Subject(s) - fusarium culmorum , incubation , mycotoxin , fusarium , food science , incubation period , substrate (aquarium) , agar , agar plate , biology , horticulture , chemistry , zoology , bacteria , ecology , biochemistry , genetics
Aims: To determine the effect of interacting conditions of water activity ( a w , 0·99–0·85), temperature (15, 25°C) and time (40 days) on growth and production of the mycotoxins deoxynivalenol (DON) and nivalenol (NIV) by Fusarium culmorum on a wheat‐based agar medium. Methods and Results: Fusarium culmorum grew optimally at 0·995 a w and minimally at 0·90 at both 15 and 25°C. No growth was observed at <0·90 a w . Overall, temperature, a w and their interaction had a statistically significant effect on the growth rate of F. culmorum . Production of both DON and NIV were over a much narrower range (0·995–0·95 a w ) than that for growth. The highest concentrations of DON and NIV levels were produced at 0·995 a w and 0·981 a w at 25°C, respectively, after 40 days of incubation. Statistically, a w , temperature and incubation time, and a w × temperature and temperature × incubation time had a statistically significant effect on DON/NIV production. Conclusions: This is the first detailed report on the two‐dimensional environmental profiles for DON/NIV production by F. culmorum in the UK. Significance and Impact of the Study: As part of a hazard analysis critical control point (HACCP) approach, this type of information is critical in monitoring critical control points for prevention of DON/NIV entering the wheat production chain.