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A sensitive and rapid method to determine the viability of freeze‐dried bacterial cells
Author(s) -
Parthuisot N.,
Catala P.,
Lebaron P.,
Clermont D.,
Bizet C.
Publication year - 2003
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2003.01335.x
Subject(s) - flow cytometry , esterase , viability assay , cytometry , biology , microbiology and biotechnology , cell counting , bacteria , escherichia coli , chromatography , food science , chemistry , cell , biochemistry , enzyme , genetics , cell cycle , gene
Aims: To determine the potential use of flow cytometry for viability asssessment of freeze‐dried bacterial cells. Methods and Results: Escherichia coli CIP 54.8T and Vibrio metschnikovii CIP 104262 were analysed. The viability of freeze‐dried cells resuspended in a nutrient broth was evaluated by culture whereas activity was determined by flow cytometry analysis of both esterase activity and cell death. Activity assessment by flow cytometry was found to be a rapid and good indicator of cell viability and was very efficient for quality control. For V. metschnikovii the fraction of active cells varies greatly depending on the freeze‐drying procedure and within a given procedure. Conclusions, Significance and Impact of the Study: Bacterial activity assessment by flow cytometry is very efficient for the control of freeze‐dried cells.