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Identification of heterofermentative lactobacilli isolated from pig faeces by numerical analysis of total soluble cell protein patterns and RAPD‐PCR
Author(s) -
Toit M. Du,
Dicks L.M.T.,
Holzapfel W.H.
Publication year - 2003
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2003.01334.x
Subject(s) - biology , rapd , feces , lactobacillus reuteri , microbiology and biotechnology , lactobacillus , lactobacillaceae , lactobacillus buchneri , bacteria , lactic acid , genetics , population , lactobacillus plantarum , genetic diversity , demography , sociology
Aims: The study deals with a number of heterofermentative Lactobacillus strains isolated from pig faeces and their identification. Methods and Results: SDS‐PAGE of total soluble cell proteins and RAPD‐PCR profiles were used to identify the strains isolated from pig faeces. Protein profiles obtained with SDS‐PAGE revealed that 15 strains clustered at r ≥ 0·78 with Lactobacillus buchneri and nine strains at r ≥ 0·77 with two reference strains of Lactobacillus reuteri . The identity of the strains was confirmed with RAPD‐PCR. Conclusions: Numerical analysis of protein profiles and RAPD‐PCR proved valuable in the differentiation of Lactobacillus spp. isolated from pig faeces. Significance: This is the first report on the association of Lact. buchneri with pig faeces.