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Effect of phenolic monomers on biomass and hydrolytic enzyme activities of an anaerobic fungus isolated from wild nil gai ( Baselophus tragocamelus )
Author(s) -
Paul S.S.,
Kamra D.N.,
Sastry V.R.B.,
Sahu N.P.,
Kumar A.
Publication year - 2003
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2003.01331.x
Subject(s) - ferulic acid , vanillic acid , rumen , lignin , food science , biology , xylanase , cellulase , anaerobic exercise , biochemistry , botany , hydrolysis , enzyme , fermentation , physiology
Aims: To test the anaerobic fungus, Piromyces sp. FNG5, for its tolerance to phenolic monomers released in the rumen by degradation of lignocellulosic poor‐quality feeds. Methods and Results: Effects of phenolic monomers on biomass and fibrolytic enzyme activities of a pure culture of lignocellulolytic anaerobic fungus ( Piromyces sp. FNG5) isolated from faeces of wild nil gai (blue bull, Baselophus tragocamelus ) were evaluated. There was a reduction in fungal biomass at 1 m m concentration of catechol with complete inhibition at 10 m m . p ‐Coumaric acid caused a reduction in biomass at 10 m m and no growth was observed above 20 m m concentration. The fungal isolate could tolerate up to 5 m m of ferulic acid without any reduction in biomass level, and was able to grow to some extent up to the highest level of ferulic acid tested (20 m m ). Vanillic acid had no effect on biomass of the fungus even up to 50 m m level. The phenolic monomers varied in their potential to inhibit the secretion of carboxymethyl cellulase, xylanase, β ‐glucosidase and acetyl esterase activities with catechol being the most inhibitory and vanillic acid being the least inhibitory. After 14 days of incubation, 38·49–65·14% p ‐Coumaric acid, 65·22–74·10% ferulic acid and 34·13–66·78% vanillic acid disappeared from the medium under anaerobic conditions. Conclusions, Significance and Impact of the Study: It is concluded that the anaerobic fungus Piromyces sp. FNG5 is tolerant to phenolic monomers and has ability to degrade them. Therefore, such anaerobic fungi may play an important role in fibre degradation in the rumen.