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Isolation and characterization of phorate degrading soil bacteria of environmental and agronomic significance
Author(s) -
Bano N.,
Musarrat J.
Publication year - 2003
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2003.01329.x
Subject(s) - isolation (microbiology) , agriculture , phorate , clinical microbiology , library science , microbiology and biotechnology , geography , biology , archaeology , ecology , computer science , pesticide
Phorate [ O , O ‐diethyl‐ S ‐(ethylthio)methyl phosphoradiothioate] degrading bacteria were isolated from agricultural soil and characterized based on their morphological and biochemical characteristics. The selected isolates PS‐1, PS‐2 and PS‐3 were presumptively identified as Rhizobium , Pseudomonas and Proteous species, respectively. The HPLC analysis of phorate in bioaugmented soil revealed its complete disappearance within 40 days. The degradation isotherms of the isolates PS‐1, PS‐2 and PS‐3 suggested time‐dependent disappearance of phorate following the first order rate kinetics at the corresponding rate constants of 0·04, 0·05 and 0·04 days −1 . Besides, the isolates concurrently exhibited substantial phosphate solubilization, indole acetic acid, and siderophore production. The isolate PS‐3 also showed anti‐fungal activity against a phytopathogen Fusarium oxysporum . As a result of the multifarious biological properties, the isolates have been suggested to be important bioresource for efficient bioinoculant development.