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Isolation and characterization of a transposon mutant of Pseudomonas aeruginosa affecting uptake of dibenzothiophene in n ‐tetradecane
Author(s) -
Noda K.,
Watanabe K.,
Maruhashi K.
Publication year - 2003
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2003.01322.x
Subject(s) - dibenzothiophene , pseudomonas aeruginosa , transposable element , tetradecane , mutant , rhodococcus , pseudomonadaceae , biology , chemistry , pseudomonadales , microbiology and biotechnology , biochemistry , bacteria , genetics , gene , organic chemistry , catalysis
Aims: Isolation and characterization of a transposon mutant of Pseudomonas aeruginosa affecting the uptake of dibenzothiophene (DBT) in n ‐tetradecane ( n ‐TD). Methods and Results: The dsz desulphurization gene cluster from Rhodococcus erythropolis KA2‐5‐1 was transferred to the chromosome of P. aeruginosa NCIMB9571 using a transposon vector. A recombinant (named PARM1) was obtained which was able to desulphurize DBT in water, but not in n ‐TD. Conclusions: PARM1 is a mutant deficient in a DBT transport system operational in n ‐TD. This transport system is independent of rhamnolipids and of the n ‐alkane transport system. Significance and Impact of the Study: Pseudomonas aeruginosa NCIMB9571 seems to have a specific system of transporting hydrophobic compounds such as DBT in oil.