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Fluorescent Pseudomonas mainly produce the dihydroform of pyoverdine at low specific growth rate
Author(s) -
Jacques P,
Ongena M,
Bernard F,
Fuchs R,
Budzikiewicz H,
Thonart P
Publication year - 2003
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2003.01304.x
Subject(s) - chemostat , pyoverdine , pseudomonas putida , pseudomonas fluorescens , chemistry , fluorescence , dilution , pseudomonadaceae , pseudomonadales , growth rate , hydrolysis , bacterial growth , siderophore , bacteria , nuclear chemistry , biochemistry , pseudomonas aeruginosa , microbiology and biotechnology , biology , enzyme , genetics , physics , geometry , mathematics , quantum mechanics , gene , thermodynamics
Aims: To analyse the influence of cell growth rate and iron concentration on the production of pyoverdines (PVDs) and of their reduced dihydro forms by three fluorescent Pseudomonas strains ( P. putida BTP16, P. fluorescens BTP7 and P. aeruginosa 7NSK2). Methods: PVD and dihydropyoverdine (DHPVD) productions were determined by LC ESI‐MS and spectrophotometry during batch and chemostat culture at different dilution rates. Significance: The relatively high PVD‐to‐DHPVD ratio (0·57) observed in pH‐controlled batch cultures suggested that a base‐catalysed chemical oxidation of the dihydroform is not the prime mechanism involved in generating PVDs. Interestingly, in chemostat cultures the PVD‐to‐DHPVD ratio was significantly reduced at low specific growth rate. Our results suggest that the oxidation of DHPVD to PVD is catalysed by an iron‐dependent enzymatic reaction rather than a chemical oxidation.