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An improved selective isolation medium for the recovery of Listeria monocytogenes from smoked fish
Author(s) -
Neamatallah A.A.N.,
Dewar S.J.,
Austin B.
Publication year - 2003
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2003.01298.x
Subject(s) - listeria monocytogenes , haddock , bacteria , nalidixic acid , food science , smoked fish , microbiology and biotechnology , biology , listeria , agar , biopreservation , antimicrobial , chemistry , fish <actinopterygii> , antibiotics , bacteriocin , antibiotic resistance , fishery , genetics
Aims: The aim of this study was to improve the selective isolation of Listeria monocytogenes from smoked haddock fillets. Methods and Results: Listeria selective agar (LSA) – Oxford formulation was supplemented with 25 μ g ml −1 of colistin sulphate and 30 μ g ml −1 of nalidixic acid. Inocula from four smoked haddock fillets produced colonies (approx. 2–13 bacteria g −1 ), identified as L. monocytogenes , on LSA supplemented with antimicrobial compounds (MLSA). Moreover, there was only negligible evidence of bacteria which were not L. monocytogenes on MLSA. In contrast, LSA supported dense bacterial growth, which was not equated with L. monocytogenes . Significance and Impact of the Study: The modified medium permitted the recovery of L. monocytogenes from smoked haddock fillets and reduced the growth of contaminating bacteria.