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Identification of a novel insertion sequence in vanB2 ‐containing Enterococcus faecium
Author(s) -
Lee W.G.,
Kim W.
Publication year - 2003
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2003.01292.x
Subject(s) - enterococcus faecium , clinical microbiology , medicine , general surgery , sequence (biology) , identification (biology) , university hospital , library science , gram positive bacterial infections , microbiology and biotechnology , biology , genetics , antibiotics , botany , computer science
Aims: The characterization of a novel insertion sequence (IS) in vanB2 ‐containing Enterococcus faecium was conducted. Methods and Results: Direct PCR amplification of ORFC region of Tn 5382 from DNA extracted from vanB2 ‐containing E. faecium , and sequence analysis were performed. A novel IS was identified. It is 1418 bp in length and contains one putative open reading frame that is similar to transposase. There exists inverted terminal repeats of 12 bp, but direct repeats are not present. According to high similarity to putative transposases of IS 3 members, such as, IS 150 , IS 861 , IS 1077 and IS 911 , we designated it ISEnfa 3 . Significance and Impact of Study: Since ISEnfa 3 was detected in all vanB2 ‐containing strains examined so far, it could be used as a tool for epidemiological study.