Premium
Control of pyrimidine synthesis in Pseudomonas fragi
Author(s) -
West T.P.
Publication year - 2002
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2002.01203.x
Subject(s) - aspartate carbamoyltransferase , pyrimidine metabolism , pyrimidine , biochemistry , purine , pseudomonas putida , pseudomonas fluorescens , enzyme , biology , bacteria , allosteric regulation , genetics
Aims:To study the regulation of thede novopyrimidine biosynthetic enzymes in the food‐spoilage agentPseudomonas fragiATCC 4973.
Methods and Results:Thede novopyrimidine biosynthetic enzymes were measured in extracts ofPs.fragiATCC 4973 cells and of cells from auxotrophs deficient for dihydroorotase or OMP decarboxylase activity. Pyrimidine biosynthetic enzyme activities in ATCC 4973 were influenced by pyrimidine supplementation to the culture medium. The pyrimidine limitation of each auxotroph elevated thede novoenzyme activities, indicating that this pathway may be repressible by a pyrimidine‐related compound. Aspartate transcarbamoylase activity in ATCC 4973 was inhibitedin vitroby pyrophosphate and purine or pyrimidine nucleotides.
Conclusions:Pyrimidine synthesis inPs.fragiappeared to be controlled at the transcriptional level and at the level of activity for aspartate transcarbamoylase. Its transcriptional regulation seemed to be more highly controlled than what was observed in the closely related speciesPseudomonas putidaandPseudomonas fluorescens.
Significance and Impact of the Study:This study demonstrates that pyrimidine synthesis is regulated inPs. fragi . This could prove useful to future studies examining its biological control and its taxonomic assignment.