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Preparation of microparticulate β‐glucan from Saccharomyces cerevisiae for use in immune potentiation
Author(s) -
Hunter K.W.,
Gault R.A.,
Berner M.D.
Publication year - 2002
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2002.01201.x
Subject(s) - sonication , glucan , saccharomyces cerevisiae , yeast , chemistry , phagocytosis , homogeneous , beta glucan , suspension (topology) , immune system , biochemistry , chromatography , microbiology and biotechnology , biology , immunology , physics , mathematics , homotopy , pure mathematics , thermodynamics
Aims: To develop a method for the preparation of an immunologically active, homogeneous, nonaggregated, microparticulate β‐glucan‐containing material from the budding yeast Saccharomyces cerevisiae .
Methods and Results: Using a combination of sonication and spray‐drying, a homogeneous preparation of 1–2‐µ diameter β‐glucan‐containing particles was made from alkali‐ and acid‐insoluble yeast cell wall material. This microparticulate β‐glucan remained in suspension longer and, following oral administration at 0·1 mg kg −1 for 14 d, enhanced phagocytosis of mouse peritoneal macrophages significantly better than did aggregated β‐glucan particles.
Conclusions: A new sonication and spray‐drying method can be employed to overcome the problem of aggregation of β‐glucan microparticles in aqueous media.
Significance and Impact of the Study: A microparticulate form of β‐glucan that remains in suspension longer for pharmaceutical applications and has superior immune potentiation characteristics has been developed.