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Effect of glutamate on arachidonic acid production from Mortierella alpina
Author(s) -
Lan W.Z.,
Qin W.M.,
Yu L.J.
Publication year - 2002
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2002.01195.x
Subject(s) - arachidonic acid , linoleic acid , biochemistry , oleic acid , biology , linolenic acid , fatty acid , pentose phosphate pathway , polyunsaturated fatty acid , chemistry , food science , metabolism , enzyme , glycolysis
Aims: To evaluate the effect of glutamate on arachidonic acid production from Mortierella alpina .
Methods and Results: Cell growth, arachidonic acid production, proportions of poly‐unsaturated fatty acids (PuFAs) in fatty acids and glucose‐6‐phosphate dehydrogenase (G6PDH) activity were analysed when glutamate concentration was 0·8 g l −1 . Biomass and arachidonic acid production were higher in the culture containing glutamate than those in the control culture, and both reached their maximum of 25 g l −1 and 1·4 g l – 1 after 7 d, respectively. The proportions of some PuFAs, oleic acid, γ ‐linolenic acid and dihomo‐ γ ‐linolenic acid were decreased while linoleic acid and arachidonic acid were enhanced by glutamate addition. Glutamate addition enhanced G6PDH activity compared with the control during the whole culture process.
Conclusions: Addition of 0·8 g l −1 glutamate was beneficial to enhance arachidonic acid production from Mortierella alpina , which was a result of activating the pentose phosphate pathway (PPP).
Significance and Impact of the Study : This study shows that the addition of glutamate and regulation of PPP had a positive influence on arachidonic acid synthesis in Mortierella species.