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Are ruminal bacteria protected against environmental stress by plant antioxidants?
Author(s) -
Holovská K.,
Lenártová V.,
Holovská K.,
Pristaš P.,
Javorský P.
Publication year - 2002
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2002.01185.x
Subject(s) - bacteria , environmental stress , biology , food science , botany , ecology , genetics
Aims: To investigate the activity response of the antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GSHPx) of the rumen bacterium Streptococcus bovis following exposure to mercury(II) chloride (HgCl 2 ) in the presence of plant antioxidants.
Methods and Results: Streptococcus bovis was grown with 0 or 5 μg ml −1 of HgCl 2 alone or together with antioxidant substances (AOS): seleno‐ l ‐methionine (Se), α‐tocopherol (αtoc), β‐carotene (βcar), melatonin (mel). The activities of SOD and GHPx were estimated in supernatants of disrupted bacterial cells. A significant decrease in the Strep. bovis SOD activity in the presence of HgCl 2 and tested AOS, except mel, was observed. The GSHPx activity of Strep. bovis was under the same cultivation conditions nonsignificantly changed and a significant decrease in the GSHPx activity was recorded only in the presence of βcar.
Conclusions: The positive effect of Se, αtoc and βcar on the elimination of environmental stress, evoked by mercury, in ruminal bacterium Strep. bovis in vitro was documented.
Significance and Impact of the Study: The potential role of plant antioxidants in elimination of the environmental stress of ruminal bacteria evoked by heavy metals is discussed.