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The characterization of oil‐degrading microorganisms from lubricating oil contaminated (scale) soil
Author(s) -
Jirasripongpun K.
Publication year - 2002
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2002.01184.x
Subject(s) - microorganism , biodegradation , contamination , nocardia , mineral oil , soil contamination , food science , chemistry , biology , environmental chemistry , pulp and paper industry , bacteria , organic chemistry , ecology , genetics , engineering
Aims: To isolate and characterize oil‐degrading microorganisms from contaminated (scale) soil.
Methods and Results: Oil‐degrading microorganisms were isolated from enrichment cultures of scale soil. Each isolate was identified using 16S rDNA gene and oil degradability was determined on both unused and used lubricating oil. The weight of the extracted remaining oil revealed that most isolates degraded unused lubricating oil more than used lubricating oil. Chemical composition of oil analysed by TLC‐FID and GC‐MS demonstrated that Nocardia simplex W9 degraded used oil the best, and resulted in a decrease in saturates, aromatics and resins to 52·46, 38·13 and 18·81%, respectively.
Conclusions: Nocardia simplex W9 is the best degrader, among all the isolates, on both used and unused lubricating oil.
Significance and Impact of the Study: The presence of Nocardia simplex W9 in scale soil enables iron to be recycled by biodegradation.