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Identification and sequence of an unstable DNA element in the entomopathogenic bacteria Photorhabdus temperata strain K122
Author(s) -
Roche D.M.,
Dowds B.C.A.
Publication year - 2002
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2002.01158.x
Subject(s) - photorhabdus , biology , amplified fragment length polymorphism , bacteria , genetics , gene , microbiology and biotechnology , whole genome sequencing , strain (injury) , escherichia coli , dna , restriction fragment , restriction fragment length polymorphism , photorhabdus luminescens , dna sequencing , genome , polymerase chain reaction , genetic diversity , population , demography , anatomy , sociology
Aims: A search was conducted for a difference in genome composition between phenotypic variants of the insect pathogenic bacteria, Photorhabdus temperata . Methods: An unstable 300 bp fragment of DNA was identified by amplified fragment length polymorphism (AFLP) analysis, which was not, however, associated with phenotypic variation. Results: During prolonged culturing of the bacteria, one copy of the repeated fragment was deleted and a restriction site linked to one of the copies was lost or gained. The sequence did not show substantial identity to any in the database, but a 16‐bp region was identical to part of the mar R gene of Escherichia coli . Significance and Impact of the Study: The work has implications for the understanding of genetic instability in this and other pathogenic species of bacteria. In addition, the complete unstable element may be useful as a genetic tool in Photorhabdus spp.