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Identification of Lactobacillus crispatus by polymerase chain reaction targeting S‐layer protein gene
Author(s) -
Horie M.,
Kajikawa H.S.,
Toba T.
Publication year - 2002
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2002.01141.x
Subject(s) - lactobacillus crispatus , primer (cosmetics) , s layer , polymerase chain reaction , biology , taq polymerase , polymerase , gene , microbiology and biotechnology , genetics , lactobacillus , bacteria , chemistry , thermus aquaticus , organic chemistry
Aims: This study aimed to develop a polymerase chain reaction (PCR) method to identifyLactobacillus crispatus . Methods and Results: A primer set (CbsA2F–CbsA2R) for amplifying conserved regions of S‐layer genes was designed to identifyLact. crispatusand the specificity of this set was compared with that of another primer set (Cri 16SI–Cri 16SII) which has been reported as a species‐specific primer set targeting the 16S rRNA gene. Among species in theLact. acidophilusA1–A4 groups, when KOD polymerase was used for amplification, the primer set CbsA2F–CbsA2R gave PCR products withLact. crispatusstrains only. However, whenTaqpolymerase was used, this primer set gave products with oneLact. amylovorusstrain as well as withLact. crispatusstrains. The primer set Cri 16SI–Cri 16SII gave PCR products withLact. crispatusstrains and twoLact. acidophilusstrains, regardless of whether the polymerase used was KOD orTaq . Conclusions: A PCR targeting the S‐layer gene and amplified with KOD polymerase can identifyLact. crispatus accurately and rapidly. Significance and Impact of the Study: To the authors' knowledge, this is the first paper to provide a PCR method for the specific identification ofLact. crispatus .