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Identification and characterization of an oligopeptide transport system in Leuconostoc mesenteroides subsp. mesenteroides CNRZ 1463
Author(s) -
GermainAlpettaz V.,
FoucaudScheunemann C.
Publication year - 2002
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2002.01130.x
Subject(s) - leuconostoc mesenteroides , oligopeptide , biochemistry , chemistry , amino acid , lactic acid , biology , bacteria , peptide , genetics
Aims: To identify and characterize an oligopeptide transport system inLeuconostoc mesenteroides CNRZ 1473. Methods and Results: The uptake of a model substrate was monitored by determining intracellular concentrations of the corresponding amino acids by means of reversed‐phase HPLC analysis. The oligopeptide transport system is specific for peptides containing at least four amino acid residues and operative under physiological conditions of growth. It is expressed maximally in the presence of oligopeptides, enhanced in the presence of Mg2+or Ca2+ ions, and driven by ATP or a related energy‐rich phosphorylated intermediate. Conclusions: The study showed evidence for and characterized the oligopeptide transport system ofLeuc. mesenteroides for the first time. Significance and Impact of the Study: The potential of the findings is discussed with reference to the growth ofLeuc. mesenteroides in mixed‐strain cultures for the dairy industry.