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Typing of human and bovine Staphylococcus aureus by RAPD‐PCR and ribotyping‐PCR
Author(s) -
Pereira M.S.V.,
Leal N.C.,
Leal T.C.A.,
Sobreira M.,
De Almeida A.M.P.,
SiqueiraJúnior J.P.,
CamposTakaki G.M.
Publication year - 2002
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2002.01127.x
Subject(s) - typing , rapd , library science , biology , microbiology and biotechnology , sociology , demography , population , computer science , genetic diversity
Aims: To investigate genetic diversity amongStaphylococcus aureus and to delineate the geographical distribution of the strains found. Methods and Results: RAPD‐PCR and ribotyping‐PCR were employed for the characterization ofStaph. aureus isolates from bovine and nosocomial origin. Among the strains, five to nine groups were distinguished by RAPD‐PCR, depending on which primer was used, while ribotyping‐PCR distinguished seven ribotypes. Conclusions, and Significance and Impact of the Study: These results demonstrate the genetic heterogeneity of the strains studied, and the large dissemination of some clones throughout different regions and hosts, findings that may allow the monitoring ofStaph. aureus infections.