Premium
Effect of diethylsulphoxide on growth, survival and ion exchange of Escherichia coli
Author(s) -
Markarian S.A.,
Poladyan A.A.,
Kirakosyan G.R.,
Trchounian A.A.,
Bagramyan K.A.
Publication year - 2002
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2002.01112.x
Subject(s) - escherichia coli , anaerobic exercise , chemistry , ionic bonding , bacterial growth , ion exchange , electron acceptor , nuclear chemistry , medicinal chemistry , bacteria , biology , ion , biochemistry , organic chemistry , physiology , genetics , gene
Aims: To study the effect of diethylsulphoxide (DESO) on Escherichia coli growth, survival and ionic exchange in comparison with dimethylsulphoxide (DMSO). Methods and Results: Bacterial survival was estimated by counting colony‐forming units and by the most probable number (five‐tube) technique; the K + and H + transport and H 2 formation were determined electrochemically. Diethylsulphoxide at concentrations between 0·01 and 0·5% (w/v) stimulated and above 5% decreased the anaerobic growth rate and survival. 2H + : K + exchange and H 2 formation were lost at 5% DESO. At 0·05% DESO the kinetic characteristics of H + : K + exchange and H 2 formation were typical for Δμ H+ ‐dependent TrkA uncoupled with F 0 F 1 under respiration. Conclusions: Diethylsulphoxide at low concentrations serves as an electron acceptor for an anaerobic respiratory chain stimulating bacterial growth and survival through the modulation of H + : K + exchange and H 2 formation activity. The effects of DESO were more pronounced than those of DMSO. Significance and Impact of the Study: Diethylsulphoxide determines essential biological and therapeutic properties that make its application preferable.