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Improved polygalacturonase production from Bacillus sp. MG‐cp‐2 under submerged (SmF) and solid state (SSF) fermentation
Author(s) -
Kapoor M.,
Kuhad R.C.
Publication year - 2002
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2002.01107.x
Subject(s) - new delhi , pectinase , library science , fermentation , biology , geography , food science , archaeology , computer science , biochemistry , enzyme , metropolitan area
Aims: To investigate the effect of amino acids, vitamins and surfactants on polygalacturonase production from Bacillus sp. MG‐cp‐2 under submerged (SmF) and solid state fermentation (SSF). Methods and Results: Bacillus sp. MG‐cp‐2 was isolated from the outer covering of the seeds of Celastrus paniculatus . Out of the various surfactants, amino acids and vitamins, Tween‐60, DL ‐serine and folic acid maximally enhanced polygalacturonase production by 2·7‐fold (240·0 U ml −1 ), 4·0‐fold (360·0 U ml −1 ) and 3·8‐fold (342·0 U ml −1 ) respectively, under submerged fermentation (SmF). In solid state fermentation (SSF), Tween‐80, pyridoxine and DL ‐ornithine monohydrochloride induced highest enzyme production up to 1·73‐fold (6956·5 U g −1 ), 5·3‐fold (21224·4 U g −1 ) and 5·74‐fold (23076·9 U g −1 ), respectively. Conclusion: Amino acids and their analogues, vitamins and surfactants effect significantly polygalacturonase production by Bacillus sp. MG‐cp‐2 when grown under submerged (SmF) and solid state fermentation (SSF) conditions. Significance and Impact of the Study: The study provides useful information about regulation of polygalacturonase biosynthesis in Bacillus sp. MG‐cp‐2, which appears to be an interplay of nutritional and physical factors. Alkaline polygalacturonase from Bacillus sp. MG‐cp‐2 will be extremely useful in the treatment of alkaline pectic waste waters from vegetable and fruit processing industries and in degumming of bast fibres.