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Influence of nutritional conditions on the mycelial growth and exopolysaccharide production in Paecilomyces sinclairii
Author(s) -
Kim S.W.,
Hwang H.J.,
Xu C.P.,
Na Y.S.,
Song S.K.,
Yun J.W.
Publication year - 2002
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2002.01105.x
Subject(s) - industrial fermentation , paecilomyces , mycelium , sucrose , food science , biology , nitrogen , yield (engineering) , fermentation , liquid culture , carbon source , chemistry , botany , biochemistry , materials science , organic chemistry , metallurgy
Aims: The objective of the study was to optimize the submerged culture conditions for the production of exopolysaccharide from Paecilomyces sinclairii . Methods and Results: The optimal temperature and initial pH for exopolysaccharide production by Paecilomyces sinclairii in shake flask culture were found to be 30°C and 6·0, respectively. Sucrose (60 g l −1 ) and corn steep powder (10 g l −1 ) were the most suitable carbon and nitrogen source for exopolysaccharide production. Conclusions: Under optimal culture medium, the maximum exopolysaccharide concentration in a 5‐l stirred‐tank fermenter indicated 7·4 g l −1 , which was approximately three times higher than that in basal medium. The maximum specific growth rates (μ max ) and yield coefficient (Y P/S ) in the optimal culture medium was 0·16 h −1 and 0·19, respectively. Significance and Impact of the Study: The optimal culture conditions reported in this article can be widely applied to the processes for submerged cultures of other mushrooms.