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PCR/restriction fragment length polymorphism (RFLP) typing of human and poultry Campylobacter jejuni strains
Author(s) -
Steinhauserova I.,
Ceskova J.,
Nebola M.
Publication year - 2002
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2002.01096.x
Subject(s) - biology , campylobacter , campylobacter jejuni , typing , restriction fragment length polymorphism , haeiii , microbiology and biotechnology , restriction enzyme , campylobacteriosis , campylobacter coli , genetics , genotype , bacteria , gene
Aims: The PCR/RFLP typing of 156 isolates Campylobacter jejuni originating from poultry and humans was performed (101 human and 55 poultry strains). Methods and Results: On the basis of restrictive digest, six types were identified with AfaI , seven types with MboI and five types with HaeIII . With a combination of these three enzymes, 22 types were found. In human strains, the most frequently occurring types were Cj.4 (28%), Cj.1 (19%), Cj. 13 (13%) and Cj. 2 (5%). In the case of poultry strains, the most frequent types were Cj. 1 (34%), Cj. 11 (22%), C.j. 21 (16%) and Cj. 15 (11%). Conclusions: The findings support the hypothesis that poultry is a significant source but not sole source of Campylobacter sp. in relation to humans. Significance and Impact of the Study: The typing of Campylobacter sp. forms the basis for an evaluation of the current state and risk assessment of various Campylobacter sp. sources in relation to humans.