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Manipulation of temperature and illumination conditions for enhanced β‐carotene production by mutant 32 of Rhodotorula glutinis
Author(s) -
Bhosale P.,
Gadre R.V.
Publication year - 2002
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2002.01095.x
Subject(s) - industrial fermentation , carotenoid , beta carotene , rhodotorula , food science , pigment , fermentation , carotene , chemistry , biology , biochemistry , yeast , organic chemistry
Aims: Enhancement in the production of β‐carotene by the hyper producer mutant 32 of Rhodotorula glutinis by manipulation of temperature and illumination. Methods and Results: Growth and β‐carotene production was investigated in a 1 litre fermenter at different temperature and illumination conditions. The optimum temperature for growth and β‐carotene production was 30 and 20°C, respectively. At 30°C, β‐carotene production was 125 ± 2 mg l −1 and accounted for 66% of the total carotenoids in 72 h; at 20°C, it was 250 ± 7 mg l −1 and accounted for 92% of total carotenoid content. Continuous illumination of the fermenter by 1000 lx white light hampered growth as well as carotenoid synthesis. At 30°C, illuminating the fermenter in late logarithmic phase resulted in a 58% increase in β‐carotene production with a concurrent decrease in torulene; at 20°C, however, it showed no appreciable increase. Significance and Impact of the Study: Proper manipulation of culture conditions enhanced β‐carotene production by R. glutinis which makes it a significant source of β‐carotene.