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Antibiotic susceptibility of bacterial strains isolated from patients with various infections
Author(s) -
Lee S.H.,
Jeong S.H.
Publication year - 2002
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2002.01088.x
Subject(s) - microbiology and biotechnology , cephalosporin , biology , klebsiella pneumoniae , enterobacter cloacae , beta lactamase , antibiotics , multiple drug resistance , antibiotic resistance , enterobacter , gene , escherichia coli , virology , genetics
Aims: Isolates from various samples obtained during 1998 and 1999 were identified and their susceptibility to third‐generation cephalosporins, monobactams and/or cephamycins studied along with any production of ESBLs. Methods and Results: Of these samples, bacteria most frequently isolated by the conventional techniques and Vitek GNI card were Escherichia coli (37%), Klebsiella pneumoniae (27%) and Enterobacter cloacae (16%). Using disk diffusion and double‐disk synergy tests, we found that 71% strains produced ESBLs and 18% strains produced ESBLs and cephamycinases. Banding patterns of PCR amplification with the designed primers showed that 57% strains were capable of harbouring bla SHV genes. The bla TEM , bla CMY and bla AmpC genes were harboured by 55%, 31% and 12% strains, respectively. Forty‐five percent of strains contained more than two types of β‐lactamase genes. In particular, one strain contained bla TEM , bla SHV , bla CMY and bla AmpC genes. Conclusions: The percentage of ESBL‐producing strains was high. The most prevalent β‐lactamase gene was bla SHV gene. The bla CMY genes have been prevalent in cephamycin‐resistant strains. The multidrug‐resistant strains resistant to third‐generation cephalosporins and cephamycins were detected in high percentage. Significance and Impact of the Study: Resistance mechanisms to β‐lactams, comprising mostly extended‐spectrum β‐lactamase (ESBL) production, lead to the resistance against even recently developed β‐lactams in enterobacteria, which is now a serious threat to antibiotic therapy. The high prevalence of bla CMY genes and multidrug‐resistant genes may also cause therapeutic failure and lack of eradiation of these strains by third‐generation cephalosporins or cephamycins.